Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
Product Information
55002-1-AP targets NOVA2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | neuro-oncological ventral antigen 2 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 50-55 kDa, 65-70 kDa |
| GenBank accession number | NM_002516 |
| Gene Symbol | NOVA2 |
| Gene ID (NCBI) | 4858 |
| RRID | AB_10858926 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNW9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOVA2 also named as ANOVA and NOVA3. It regulates RNA splicing or metabolism in a specific subset of developing neurons. NOVA2 binds single strand RNA. The antibody is specific to NOVA2. By the alternative splicing, NOVA2 can be detected as 50-55 and 70-80 kDa (PMID:26446569, PMID:9789075).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOVA2 antibody 55002-1-AP | Download protocol |
| IP protocol for NOVA2 antibody 55002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis. | ||
Nutrients Nova1 or Bim Deficiency in Pancreatic β-Cells Does Not Alter Multiple Low-Dose Streptozotocin-Induced Diabetes and Diet-Induced Obesity in Mice | ||
Hepatol Commun METTL14 reverses liver fibrosis by inhibiting NOVA2 through an m6A-YTHDF2-dependent mechanism | ||
medRxiv Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression | ||
Neuron Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes |