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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOX2 Recombinant antibody

NOX2 Recombinant Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B17

Cat no : 82872-1-RR

Synonyms

CYBB, EC:1.-.-.-, Cytochrome b-245 heavy chain, CGD91-phox, CGD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOX2 Recombinant antibody (82872-1-RR)

    WB analysis using 82872-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82872-1-RR (CYBB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using NOX2 Recombinant antibody (82872-1-RR)

    IF Staining of mouse spleen using 82872-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using NOX2 Recombinant antibody (82872-1-RR)

    IF Staining of mouse spleen using 82872-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using NOX2 Recombinant antibody (82872-1-RR)

    IF Staining of mouse spleen using 82872-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using NOX2 Recombinant antibody (82872-1-RR)

    IF Staining of mouse spleen using 82872-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of RAW 264.7 cells using NOX2 Recombinant antibody (82872-1-RR)

    FC experiment of RAW 264.7 using 82872-1-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug NOX2 Recombinant antibody (82872-1-RR, Clone:1B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IF-P detected inmouse spleen tissue
Positive FC (Intra) detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82872-1-RR targets NOX2 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NOX2 fusion protein Ag4402 相同性解析による交差性が予測される生物種
Full Name cytochrome b-245, beta polypeptide
Calculated molecular weight 577 aa, 65 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC032720
Gene symbol NOX2
Gene ID (NCBI) 1536
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).

Protocols

Product Specific Protocols
WB protocol for NOX2 antibody 82872-1-RRDownload protocol
IF protocol for NOX2 antibody 82872-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols