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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOX4 Monoclonal antibody, PBS Only

NOX4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E5F1

Cat no : 67681-1-PBS

Synonyms

Kidney oxidase 1, KOX, KOX 1, NADPH oxidase 4, NOX4, Renal NAD(P)H oxidase, RENOX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOX4 Monoclonal antibody (67681-1-Ig)

    WB analysis using 67681-1-Ig (same clone as 67681-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using NOX4 Monoclonal antibody (67681-1-Ig)

    WB analysis of Jurkat using 67681-1-Ig (same clone as 67681-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using NOX4 Monoclonal antibody (67681-1-Ig)

    WB analysis of U-87 MG using 67681-1-Ig (same clone as 67681-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using NOX4 Monoclonal antibody (67681-1-Ig)

    WB analysis of HSC-T6 using 67681-1-Ig (same clone as 67681-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NOX4 Monoclonal antibody (67681-1-Ig)

    IHC staining of human kidney using 67681-1-Ig (same clone as 67681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NOX4 Monoclonal antibody (67681-1-Ig)

    IHC staining of human kidney using 67681-1-Ig (same clone as 67681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using NOX4 Monoclonal antibody (67681-1-Ig)

    IF Staining of HUVEC using 67681-1-Ig (same clone as 67681-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using NOX4 Monoclonal antibody (67681-1-Ig)

    IF Staining of HUVEC using 67681-1-Ig (same clone as 67681-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67681-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67681-1-PBS targets NOX4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NOX4 fusion protein Ag6176 相同性解析による交差性が予測される生物種
Full Name NADPH oxidase 4
Calculated molecular weight 67 kDa
Observed molecular weight67 kDa
GenBank accession numberBC040105
Gene symbol NOX4
Gene ID (NCBI) 50507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage (PMID: 11728818, PMID: 29285262, PMID: 14670934). Nox4 in cardiac myocytes is primarily expressed in mitochondria, and upregulation of Nox4 induced by hypertrophic stimuli elicits mitochondrial dysfunction and cardiac failure. In breast or ovarian tumor cells, mitochondrial Nox4 contributes to oncogenesis. In vascular endothelial cells, however, Nox4 is expressed in the endoplasmic reticulum (ER) and plays a specific role in redox-mediated ER signaling (PMID: 24259511).