Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| CoIP | See 2 publications below |
Product Information
25350-1-AP targets NOX5 in WB, IHC, CoIP, ELISA, PLA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NOX5 fusion protein Ag18673 相同性解析による交差性が予測される生物種 |
| Full Name | NADPH oxidase, EF-hand calcium binding domain 5 |
| Calculated molecular weight | 765 aa, 86 kDa |
| Observed molecular weight | 82-86 kDa |
| GenBank accession number | BC125098 |
| Gene Symbol | NOX5 |
| Gene ID (NCBI) | 79400 |
| RRID | AB_2811208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PH1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOX5 (NADPH oxidase, EF-hand calcium binding domain 5) is an NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca(2+)-dependent manner. It plays an important role in PDGF-induced JAK/STAT activation and human aortic smooth muscle cell (HASMC) proliferation. NOX5 is the main source of superoxide and is implicated in human spermatozoa motility. This protein has 6 isoforms produced by alternative splicing, named NOX5-α, -β, -γ, -δ, -ε and -ζ with the molecular mass of 84, 82, 86, 85, 64 and 83 kDa (PMID: 22427510 and Uniprot).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOX5 antibody 25350-1-AP | Download protocol |
| IHC protocol for NOX5 antibody 25350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Chronic hypoxia of endothelial cells boosts HIF-1α-NLRP1 circuit in Alzheimer's disease | ||
Free Radic Biol Med Nitric oxide and heme-NO stimulate superoxide production by NADPH oxidase 5. | ||
Ecotoxicol Environ Saf Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity. | ||
Biomed Pharmacother Therapeutic effect of phycocyanin on acute liver oxidative damage caused by X-ray. | ||
Biochem Biophys Res Commun miR-485 suppresses inflammation and proliferation of mesangial cells in an in vitro model of diabetic nephropathy by targeting NOX5. |