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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using NPBWR2 Polyclonal antibody (24162-1-AP)

    IHC staining of human brain using 24162-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24162-1-AP (NPBWR2 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NPBWR2 Polyclonal antibody (24162-1-AP)

    IHC staining of human brain using 24162-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24162-1-AP (NPBWR2 Antibody) at dilution of 1:100 (under 40x lens).

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NPBWR2 Polyclonal antibody

NPBWR2 Polyclonal Antibody for IHC, ELISA
Cat No. 24162-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

G protein coupled receptor 8, GPR8, neuropeptides B/W receptor 2, NPBWR2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24162-1-AP targets NPBWR2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPBWR2 fusion protein Ag20874 相同性解析による交差性が予測される生物種
Full Name neuropeptides B/W receptor 2
Calculated molecular weight 333 aa, 37 kDa
GenBank accession numberBC067481
Gene Symbol NPBWR2
Gene ID (NCBI) 2832
RRIDAB_2879442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP48146
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPBWR1 (GPR7) and NPBWR2 (GPR8) are two structurally related G-protein coupled receptors. These two receptors share high sequence identity to each other and significant similarity with opioid and somatostatin receptors (PMID: 10407191). Neuropeptide B (NPB) and neuropeptide W (NPW) are endogenous peptide ligands of these receptors. NPBWR1 was found highly conserved in both human and rodents while NPBWR2 gene was not found in the rodent genomes (PMID: 7590751; 10407191). NPBWR1 and NPBWR2 are implicated in energy homeostasis, pain, and emotion (PMID: 23515889).

Protocols

Product Specific Protocols
IHC protocol for NPBWR2 antibody 24162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols