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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NPEPL1 Polyclonal antibody

NPEPL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17211-1-AP

Synonyms

aminopeptidase like 1, KIAA1974, NPEPL1, Probable aminopeptidase NPEPL1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPEPL1 Polyclonal antibody (17211-1-AP)

    WB analysis using 17211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NPEPL1 Polyclonal antibody (17211-1-AP)

    WB analysis of HeLa using 17211-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NPEPL1 Polyclonal antibody (17211-1-AP)

    WB analysis of MCF-7 using 17211-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NPEPL1 Polyclonal antibody (17211-1-AP)

    WB analysis of HeLa using 17211-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NPEPL1 Polyclonal antibody (17211-1-AP)

    IHC staining of human kidney using 17211-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17211-1-AP (NPEPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NPEPL1 Polyclonal antibody (17211-1-AP)

    IHC staining of human kidney using 17211-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17211-1-AP (NPEPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NPEPL1 Polyclonal antibody (17211-1-AP)

    IF Staining of MCF-7 using 17211-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NPEPL1 antibody (17211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17211-1-AP targets NPEPL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPEPL1 fusion protein Ag10994 相同性解析による交差性が予測される生物種
Full Name aminopeptidase-like 1
Calculated molecular weight 523 aa, 56 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC020507
Gene symbol NPEPL1
Gene ID (NCBI) 79716
RRIDAB_2267625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NPEPL1 antibody 17211-1-APDownload protocol
IHC protocol for NPEPL1 antibody 17211-1-APDownload protocol
IF protocol for NPEPL1 antibody 17211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols