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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPEPPS Polyclonal antibody (31633-1-AP)

    WB analysis using 31633-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31633-1-AP (NPEPPS antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using NPEPPS Polyclonal antibody (31633-1-AP)

    IF Staining of A549 using 31633-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NPEPPS antibody (31633-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NPEPPS Polyclonal antibody

NPEPPS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31633-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

AAP S, Cytosol alanyl aminopeptidase, MP100, NPEPPS, PSA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31633-1-AP targets NPEPPS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPEPPS fusion protein Ag36035 相同性解析による交差性が予測される生物種
Full Name aminopeptidase puromycin sensitive
Observed molecular weight103 kDa
GenBank accession numberBC065294
Gene Symbol NPEPPS
Gene ID (NCBI) 9520
RRIDAB_3670057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP55786
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for NPEPPS antibody 31633-1-APDownload protocol
IF protocol for NPEPPS antibody 31633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols