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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPEPPS Recombinant antibody (84230-5-RR)

    WB analysis using 84230-5-RR (same clone as 84230-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84230-5-RR (NPEPPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84230-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NPEPPS Recombinant antibody (84230-5-RR)

    IHC staining of mouse liver using 84230-5-RR (same clone as 84230-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84230-5-RR (NPEPPS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84230-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using NPEPPS Recombinant antibody (84230-5-RR)

    IF Staining of A549 using 84230-5-RR (same clone as 84230-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using NPEPPS antibody (84230-5-RR, Clone: 241502G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84230-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84230-5-RR

    Affinity and Kinetic Characterization of 84230-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84230-5-RR against Human NPEPPS were performed. The affinity constant is below 1 pM.

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NPEPPS Recombinant antibody, PBS Only

NPEPPS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84230-5-PBS
Clone No.241502G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PSA, MP100, AAP S, 241502G1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84230-5-PBS targets NPEPPS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NPEPPS fusion protein Ag36035 相同性解析による交差性が予測される生物種
Full Name aminopeptidase puromycin sensitive
Observed molecular weight103 kda
GenBank accession numberBC065294
Gene Symbol NPEPPS
Gene ID (NCBI) 9520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55786
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.