Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, Y79 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 10 publications below |
Product Information
13812-1-AP targets NPHP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NPHP4 fusion protein Ag4875 相同性解析による交差性が予測される生物種 |
| Full Name | nephronophthisis 4 |
| Calculated molecular weight | 1426 aa, 158 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC040520 |
| Gene Symbol | NPHP4 |
| Gene ID (NCBI) | 261734 |
| RRID | AB_10640302 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75161 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPHP4 antibody 13812-1-AP | Download protocol |
| IHC protocol for NPHP4 antibody 13812-1-AP | Download protocol |
| IF protocol for NPHP4 antibody 13812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. | ||
Genetics Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells. |