NPHS2 Polyclonal antibody
NPHS2 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat and More (2)
Applications
WB, IHC, IF-P, IF-Fro, ELISA
Conjugate
Unconjugated
Cat no : 20384-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat kidney tissue |
| Positive IHC detected in | rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue, zebrafish embryos |
| Positive IF-Fro detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 35 publications below |
| IHC | See 7 publications below |
| IF | See 26 publications below |
Product Information
20384-1-AP targets NPHS2 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NPHS2 fusion protein Ag14213 相同性解析による交差性が予測される生物種 |
| Full Name | nephrosis 2, idiopathic, steroid-resistant (podocin) |
| Calculated molecular weight | 383 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC029141 |
| Gene symbol | NPHS2 |
| Gene ID (NCBI) | 7827 |
| RRID | AB_10697662 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPHS2 (also known as Podocin) is a membrane protein located on the podocyte foot process and is the critical component of the glomerular filtration barrier. Mutations of NPHS2 cause recessive steroidresistant nephrotic syndrome. Two isoforms of NPHS2 exist with molecular weights of 42 kDa and 35 kDa, respectively. (PMID: 21499232)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPHS2 antibody 20384-1-AP | Download protocol |
| IHC protocol for NPHS2 antibody 20384-1-AP | Download protocol |
| IF protocol for NPHS2 antibody 20384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Kidney Int A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome | ||
Phytomedicine Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro | ||
Phytomedicine Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis | ||
Front Immunol Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy | ||
Cell Physiol Biochem Weighted Gene Correlation Network Analysis (WGCNA) Detected Loss of MAGI2 Promotes Chronic Kidney Disease (CKD) by Podocyte Damage. |
