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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

B23/NPM1 Polyclonal antibody

B23/NPM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10306-1-AP

Synonyms

B23, NPM1, Numatrin, Nucleophosmin, NPM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B23/NPM1 Polyclonal antibody (10306-1-AP)

    WB analysis using 10306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23/NPM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of multi-cells using B23/NPM1 Polyclonal antibody (10306-1-AP)

    WB analysis of multi-cells using 10306-1-AP

    WB result of anti-NPM1 (10306-1-AP) in different cell lysates.

  • Western Blot (WB) analysis of various lysates using B23/NPM1 Polyclonal antibody (10306-1-AP)

    WB analysis using 10306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23/NPM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using B23/NPM1 Polyclonal antibody (10306-1-AP)

    WB analysis of C6 using 10306-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using B23/NPM1 Polyclonal antibody (10306-1-AP)

    WB analysis of K-562 using 10306-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (NPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IP experiment of Jurkat using 10306-1-AP

    IP result of anti-B23/NPM1 (IP:10306-1-AP, 4ug; Detection:10306-1-AP 1:2000) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IHC staining of human colon cancer using 10306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IHC staining of human colon cancer using 10306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IHC staining of human normal colon using 10306-1-AP

    Immunohistochemical analysis of paraffin-embedded normal colon slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IHC staining of human colon cancer using 10306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IHC staining of human colon cancer using 10306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IHC staining of human breast cancer using 10306-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IHC staining of human breast cancer using 10306-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IF Staining of HeLa using 10306-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using B23/NPM1 antibody (10306-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using B23/NPM1 Polyclonal antibody (10306-1-AP)

    IF Staining of HeLa using 10306-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10306-1-AP (B23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inCOLO 320 cells, Jurkat cells, multi-cells, K-562 cells, HeLa cells, HEK-293 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10306-1-AP targets B23/NPM1 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B23/NPM1 fusion protein Ag0286 相同性解析による交差性が予測される生物種
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated molecular weight 33 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC002398
Gene symbol NPM1
Gene ID (NCBI) 4869
RRIDAB_2155163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Protocols

Product Specific Protocols
WB protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IHC protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IF protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IP protocol for B23/NPM1 antibody 10306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
mouse

EMBO Mol Med

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.

Authors - Masayuki Ide
View Article
humanchIP

Cancer Res

LNMICC promotes nodal metastasis of cervical cancer by reprogramming fatty acid metabolism.

Authors - Chunliang Shang
View Article
humanIF

Int J Mol Sci

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

Authors - Ahmed Hassan
View Article
humanWB,IF

Mol Ther Nucleic Acids

RPL32 Promotes Lung Cancer Progression by Facilitating p53 Degradation.

Authors - Jiansheng Xie
View Article
humanWB

iScience

Elevated high-mannose N-glycans hamper endometrial decidualization

Authors - Siyi Chen
  • KD Validated
View Article