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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NPSR1 Polyclonal antibody

NPSR1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25085-1-AP

Synonyms

neuropeptide S receptor 1, Neuropeptide S receptor, GPRA, GPR154, G protein coupled receptor 154

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NPSR1 Polyclonal antibody (25085-1-AP)

    IHC staining of human colon cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using NPSR1 Polyclonal antibody (25085-1-AP)

    IHC staining of human cervical cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using NPSR1 Polyclonal antibody (25085-1-AP)

    IHC staining of human endometrial cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25085-1-AP targets NPSR1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPSR1 fusion protein Ag18785 相同性解析による交差性が予測される生物種
Full Name neuropeptide S receptor 1
Calculated molecular weight 371 aa, 43 kDa
GenBank accession numberBC132693
Gene symbol NPSR1
Gene ID (NCBI) 387129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuropeptide S receptor 1 (NPSR1), previously known as GPRA and GPR154, is a G protein-coupled receptor (GPCR) that plays a significant role in various physiological processes. NPSR1 is primarily expressed in the bronchus, brain, and immune cells. It is also found in specific peripheral cell types such as monocytes/macrophages and neuroendocrine cells of the gut (PMID: 30711025).

Protocols

Product Specific Protocols
IHC protocol for NPSR1 antibody 25085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols