Validation Data Gallery
Tested Applications
| Positive WB detected in | pig pituitary gland tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68709-1-Ig targets NPTX2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NPTX2 fusion protein Ag33808 相同性解析による交差性が予測される生物種 |
| Full Name | neuronal pentraxin II |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC009924 |
| Gene Symbol | NPTX2 |
| Gene ID (NCBI) | 4885 |
| RRID | AB_3670402 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P47972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPTX2 antibody 68709-1-Ig | Download protocol |
| IF protocol for NPTX2 antibody 68709-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |