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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NQO1 Monoclonal antibody, PBS Only

NQO1 Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

rat, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1E5G7

Cat no : 67240-1-PBS

Synonyms

DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NQO1 Monoclonal antibody (67240-1-Ig)

    WB analysis using 67240-1-Ig (same clone as 67240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NQO1 Monoclonal antibody (67240-1-Ig)

    WB analysis of HepG2 using 67240-1-Ig (same clone as 67240-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using NQO1 Monoclonal antibody (67240-1-Ig)

    WB analysis of HSC-T6 using 67240-1-Ig (same clone as 67240-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NQO1 Monoclonal antibody (67240-1-Ig)

    IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NQO1 Monoclonal antibody (67240-1-Ig)

    IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using NQO1 Monoclonal antibody (67240-1-Ig)

    FC experiment of MCF-7 using 67240-1-Ig (same clone as 67240-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NQO1 (67240-1-Ig, Clone:1E5G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67240-1-PBS targets NQO1 in Indirect ELISA, IHC, WB applications and shows reactivity with rat, human samples.

Tested Reactivity rat, human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NQO1 fusion protein Ag28933 相同性解析による交差性が予測される生物種
Full Name NAD(P)H dehydrogenase, quinone 1
Calculated molecular weight 274 aa, 31 kDa
Observed molecular weight 29-31 kDa
GenBank accession numberBC007659
Gene symbol NQO1
Gene ID (NCBI) 1728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )