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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NQO2 Monoclonal antibody (68487-1-Ig)

    WB analysis using 68487-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit kidney tissue using NQO2 Monoclonal antibody (68487-1-Ig)

    WB analysis of rabbit kidney using 68487-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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NQO2 Monoclonal antibody

NQO2 Monoclonal Antibody for WB, ELISA
Cat No. 68487-1-Ig
Clone No.1D9B5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rabbit

Applications

WB, ELISA

Synonyms

DHQV, DIA6, NQO2, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, QR2, Quinone reductase 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, rabbit kidney tissue, HeLa, K-562, TF-1

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68487-1-Ig targets NQO2 in WB, ELISA applications and shows reactivity with Human, mouse, rabbit samples.

Tested Reactivity Human, mouse, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NQO2 fusion protein Ag8156 相同性解析による交差性が予測される生物種
Full Name NAD(P)H dehydrogenase, quinone 2
Calculated molecular weight 231 aa, 26 kDa
GenBank accession numberBC006096
Gene Symbol NQO2
Gene ID (NCBI) 4835
RRIDAB_3085198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

Protocols

Product Specific Protocols
WB protocol for NQO2 antibody 68487-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols