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NR1D1 Polyclonal antibody

Name: NR1D1 Polyclonal antibody
Brand: Proteintech
SKU: 14506-1-AP
Rating: 5.00 (2 reviews)

NR1D1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 14506-1-AP

Synonyms

Reverb alpha, Rev erbA alpha, HREV, EAR-1, EAR1

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis using 14506-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 14506-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 14506-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 14506-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 cells using 14506-1-AP
WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman.
WB analysis of A549 cells using 14506-1-AP
WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman.
IP experiment of A549 using 14506-1-AP
IP result of anti-NR1D1 (IP:14506-1-AP, 4ug; Detection:14506-1-AP 1:300) with A549 cells lysate 1200ug.
IF Staining of NIH/3T3 using 14506-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14506-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, HepG2 cells, mouse skeletal muscle tissue, Jurkat cells, NIH/3T3 cells, Y79 cells, HeLa cells
Positive IP detected in	A549 cells
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 22 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below
ChIP	See 5 publications below

Product Information

14506-1-AP targets NR1D1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NR1D1 fusion protein Ag5964 相同性解析による交差性が予測される生物種
Full Name	nuclear receptor subfamily 1, group D, member 1
Calculated molecular weight	67 kDa
Observed molecular weight	55-68 kDa
GenBank accession number	BC056148
Gene symbol	NR1D1
Gene ID (NCBI)	9572
RRID	AB_11182604
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

Protocols

Product Specific Protocols
WB protocol for NR1D1 antibody 14506-1-AP	Download protocol
IF protocol for NR1D1 antibody 14506-1-AP	Download protocol
IP protocol for NR1D1 antibody 14506-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. Authors - Yi Liu View Article
mouse	WB	Theranostics PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1. Authors - Shuai Wang View Article
human	WB,IF	Cancer Res NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer. Authors - Na-Lee Ka View Article
mouse	WB	Cell Death Discov NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome Authors - Sun Mi Kim View Article
mouse	WB	J Neuroinflammation The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome. Authors - Liang Kou View Article
human	WB	Cell Biosci Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy. Authors - Qingxue Liu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Priya (Verified Customer) (01-17-2023)	I have used for cardiomyocytes, mouse skin and liver tissues Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin
FH Satish (Verified Customer) (12-19-2019)	I got clear bands. The antibody detects two band one band around 67 kDa and another band much lower than 67kDa. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: INS1 cells

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NR1D1 Polyclonal antibody

NR1D1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14506-1-AP

WB analysis using 14506-1-AP

WB analysis using 14506-1-AP

WB analysis using 14506-1-AP

WB analysis of A549 cells using 14506-1-AP

WB analysis of A549 cells using 14506-1-AP

IP experiment of A549 using 14506-1-AP

IF Staining of NIH/3T3 using 14506-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.

NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer.

NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome

The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.

Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy.

Reviews