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NR1H3 Polyclonal antibody

NR1H3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14351-1-AP

Synonyms

RLD 1, Oxysterols receptor LXR alpha, LXRα, LXRA, LXR a


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Tested Applications

Positive WB detected inHL-60 cells, COLO 320 cells, mouse liver tissue, rat liver tissue, THP-1 cells
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14351-1-AP targets NR1H3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR1H3 fusion protein Ag5697 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 1, group H, member 3
Calculated molecular weight 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC041172
Gene symbol NR1H3
Gene ID (NCBI) 10062
RRIDAB_10640525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements. NR1H3 exists some isoforms with MV 51 kDa and 43-45 kDa.

Protocols

Product Specific Protocols
WB protocol for NR1H3 antibody 14351-1-APDownload protocol
IHC protocol for NR1H3 antibody 14351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1

Authors - Min Yan Shi
humanWB

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
mouseWB

Theranostics

Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.

Authors - Ke Feng
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
mouseWB,IF

Food Res Int

Cabernet sauvignon dry red wine ameliorates atherosclerosis in mice by regulating inflammation and endothelial function, activating AMPK phosphorylation, and modulating gut microbiota

Authors - Xinlong Cheng
human,mouseWB

Phytomedicine

Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux

Authors - Jianfei Mu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

K (Verified Customer) (06-10-2021)

works good in rat and human cells form western blotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1;1000
  • Cell Tissue Type: rat and human liver cells