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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NR1H3 Monoclonal antibody, PBS Only

NR1H3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A4F7

Cat no : 60134-1-PBS

Synonyms

Liver X receptor alpha, LXR a, LXRA, NR1H3, Oxysterols receptor LXR alpha, RLD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NR1H3 Monoclonal antibody (60134-1-Ig)

    WB analysis using 60134-1-Ig (same clone as 60134-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NR1H3 Monoclonal antibody (60134-1-Ig)

    WB analysis of HepG2 using 60134-1-Ig (same clone as 60134-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using NR1H3 Monoclonal antibody (60134-1-Ig)

    WB analysis of MCF-7 using 60134-1-Ig (same clone as 60134-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NR1H3 Monoclonal antibody (60134-1-Ig)

    IF Staining of HepG2 using 60134-1-Ig (same clone as 60134-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 60134-1-Ig (NR1H3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60134-1-PBS targets NR1H3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NR1H3 fusion protein Ag6184 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 1, group H, member 3
Calculated molecular weight 50 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC041172
Gene symbol NR1H3
Gene ID (NCBI) 10062
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.