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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NR2C1 Polyclonal antibody, PBS Only

NR2C1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13658-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NR2C1 Polyclonal antibody (13658-1-AP)

    WB analysis of SH-SY5Y using 13658-1-AP (same clone as 13658-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13658-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using NR2C1 Polyclonal antibody (13658-1-AP)

    WB analysis of PC-3 using 13658-1-AP (same clone as 13658-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NR2C1 Polyclonal antibody (13658-1-AP)

    IHC staining of human prostate cancer using 13658-1-AP (same clone as 13658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 13658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NR2C1 Polyclonal antibody (13658-1-AP)

    IHC staining of human prostate cancer using 13658-1-AP (same clone as 13658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 13658-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13658-1-PBS targets NR2C1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2C1 fusion protein Ag4554 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 2, group C, member 1
Calculated molecular weight 467 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC040141
Gene symbol NR2C1
Gene ID (NCBI) 7181
RRIDAB_2236290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear receptor subfamily 2 group C member 1 (NR2C1), also known as Orphan nuclear receptor TR2, is a member of the steroid/thyroid hormone receptor superfamily that controls a variety of processes, including growth, differentiation and development. TR2 is known to bind to regulatory elements of the erythropoietin gene, the muscle-specific aldolase A gene and the CNTF-15 gene. In addition to TR2, a related orphan receptor, designated TR4, has been identified. TR4 forms heterodimers with TR2, which are thought to be involved in neurogenesis and germ cell development. TR2 is known to be downregulated by both p53 and ionizing radiation, and it may play a role in linking p53 to members of the steroid recepr family.