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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NR2E3 Polyclonal antibody (14246-1-AP)

    WB analysis using 14246-1-AP (same clone as 14246-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using NR2E3 Polyclonal antibody (14246-1-AP)

    WB analysis of Y79 using 14246-1-AP (same clone as 14246-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NR2E3 Polyclonal antibody (14246-1-AP)

    WB analysis of HepG2 using 14246-1-AP (same clone as 14246-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NR2E3 Polyclonal antibody (14246-1-AP)

    WB analysis of HepG2 using 14246-1-AP (same clone as 14246-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14246-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NR2E3 Polyclonal antibody (14246-1-AP)

    IHC staining of human prostate cancer using 14246-1-AP (same clone as 14246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14246-1-AP (NR2E3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14246-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NR2E3 Polyclonal antibody (14246-1-AP)

    IHC staining of human prostate cancer using 14246-1-AP (same clone as 14246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14246-1-AP (NR2E3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14246-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of photoreceptor progenitor cells using NR2E3 Polyclonal antibody (14246-1-AP)

    IF Staining of photoreceptor progenitor cells using 14246-1-AP (same clone as 14246-1-PBS)

    IF result of NR2E3 antibody (14246-1-AP, 1:50) with human photoreceptor progenitors cells. This data was developed using the same antibody clone with 14246-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NR2E3 Polyclonal antibody, PBS Only

NR2E3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14246-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14246-1-PBS targets NR2E3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2E3 fusion protein Ag5503 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 2, group E, member 3
Calculated molecular weight 45 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC041421
Gene Symbol NR2E3
Gene ID (NCBI) 10002
RRIDAB_2155476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5X4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]