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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NR2E3 Monoclonal antibody

NR2E3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Pig, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G1D9

Cat no : 66494-1-Ig

Synonyms

ESCS, NR2E3, PNR, rd7, RNR, RP37

Validation Data Gallery

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  • Western Blot (WB) analysis of pig retina tissue using NR2E3 Monoclonal antibody (66494-1-Ig)

    WB analysis of pig retina using 66494-1-Ig

    pig retina tissue were subjected to SDS PAGE followed by western blot with 66494-1-Ig (NR2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse retina tissue using NR2E3 Monoclonal antibody (66494-1-Ig)

    WB analysis of mouse retina using 66494-1-Ig

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 66494-1-Ig (NR2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inpig retina tissue, mouse retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66494-1-Ig targets NR2E3 in WB, ELISA applications and shows reactivity with Pig, Mouse samples.

Tested Reactivity Pig, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NR2E3 fusion protein Ag5831 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 2, group E, member 3
Calculated molecular weight 45 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC041421
Gene symbol NR2E3
Gene ID (NCBI) 10002
RRIDAB_2881859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]

Protocols

Product Specific Protocols
WB protocol for NR2E3 antibody 66494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols