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- KD/KO Validated
NR2F1 Polyclonal antibody
NR2F1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24573-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, PC-3 cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach cancer tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
24573-1-AP targets NR2F1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NR2F1 fusion protein Ag0118 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear receptor subfamily 2, group F, member 1 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC004154 |
| Gene symbol | NR2F1 |
| Gene ID (NCBI) | 7025 |
| RRID | AB_2879616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NR2F1 (Nuclear receptor subfamily 2, group F, member 1), also known as COUP-TFI, is an orphan nuclear receptor belonging to the superfamily of steroid/thyroid hormone receptors and acting as a strong transcriptional regulator. NR2F1 has a DNA binding domain (DBD) consisting of two conserved Zinc-finger motifs and a ligand-binding domain (LBD). NR2F1 also plays an epigenetic role in mediating global histone modifications by interacting with or recruiting chromatin-remodeling enzymes. NR2F1 can be a biomarker of tumor dormancy that promotes quiescence of various types of cancer cells, including breast, prostate, and squamous cell carcinoma of the head and neck (PMID: 35740627).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NR2F1 antibody 24573-1-AP | Download protocol |
| IHC protocol for NR2F1 antibody 24573-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma. | ||
Cell Death Dis Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells. | ||
Cancers (Basel) NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer
| ||
Clin Transl Med Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma | ||
Transl Oncol Hypoxia induced cell dormancy of salivary adenoid cystic carcinoma through miR-922/DEC2 axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ryan (Verified Customer) (06-15-2018) |
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