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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NR2F6 Polyclonal antibody (15399-1-AP)

    WB analysis using 15399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15399-1-AP (NR2F6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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NR2F6 Polyclonal antibody

NR2F6 Polyclonal Antibody for WB, ELISA
Cat No. 15399-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

V-erbA-related protein 2, V erbA related protein 2, ERBAL2, EAR-2, EAR2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Raji cells, Human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15399-1-AP targets NR2F6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2F6 fusion protein Ag7614 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 2, group F, member 6
Calculated molecular weight 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC002669
Gene Symbol NR2F6
Gene ID (NCBI) 2063
RRIDAB_3669205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development (PMID:15875024). NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS)(PMID:12690040). It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity (PMID:18701084).

Protocols

Product Specific Protocols
WB protocol for NR2F6 antibody 15399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols