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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NR2F6 Monoclonal antibody (60117-2-Ig)

    WB analysis of HeLa using 60117-2-Ig (same clone as 60117-2-PBS)

    WB result of NR2F6 antibody (60117-2-Ig, 1:1000) with si-Control and si-NR2F6 transfected HeLa cells. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NR2F6 Monoclonal antibody (60117-2-Ig)

    WB analysis using 60117-2-Ig (same clone as 60117-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NR2F6 Monoclonal antibody (60117-2-Ig)

    WB analysis of HeLa using 60117-2-Ig (same clone as 60117-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NR2F6 Monoclonal antibody (60117-2-Ig)

    IHC staining of human ovary tumor using 60117-2-Ig (same clone as 60117-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NR2F6 Monoclonal antibody (60117-2-Ig)

    IHC staining of human ovary tumor using 60117-2-Ig (same clone as 60117-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NR2F6 Monoclonal antibody (60117-2-Ig)

    IHC staining of human kidney using 60117-2-Ig (same clone as 60117-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NR2F6 Monoclonal antibody (60117-2-Ig)

    IHC staining of human kidney using 60117-2-Ig (same clone as 60117-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NR2F6 Monoclonal antibody, PBS Only

NR2F6 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60117-2-PBS
Clone No.2H2B8

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2H2B8, EAR 2, EAR2, EAR-2, ERBAL2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60117-2-PBS targets NR2F6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NR2F6 fusion protein Ag7614 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 2, group F, member 6
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC002669
Gene Symbol NR2F6
Gene ID (NCBI) 2063
RRIDAB_2155653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10588
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development (PMID:15875024). NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) (PMID: 12690040). It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity (PMID:18701084).