Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 19 publications below |
| IHC | See 10 publications below |
| IF | See 7 publications below |
| ChIP | See 3 publications below |
Product Information
21854-1-AP targets NR3C2 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NR3C2 fusion protein Ag16410 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear receptor subfamily 3, group C, member 2 |
| Calculated molecular weight | 984 aa, 107 kDa |
| Observed molecular weight | 94-110 kDa |
| GenBank accession number | BC111758 |
| Gene Symbol | NR3C2 |
| Gene ID (NCBI) | 4306 |
| RRID | AB_10858785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08235 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NR3C2 antibody 21854-1-AP | Download protocol |
| IHC protocol for NR3C2 antibody 21854-1-AP | Download protocol |
| IF protocol for NR3C2 antibody 21854-1-AP | Download protocol |
| IP protocol for NR3C2 antibody 21854-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction. | ||
Oncotarget Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats. | ||
Int Immunopharmacol UUO induces lung fibrosis with macrophage-myofibroblast transition in rats. | ||
Behav Brain Res Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats. | ||
Behav Brain Res Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats. |