• Featured Product
  • KD/KO Validated

NR4A1 Polyclonal antibody

NR4A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 25851-1-AP

Synonyms

Early response protein NAK1, GFRP1, HMR, N10, NAK 1, NAK1, NGFIB, NR4A1, NUR77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST 59, Testicular receptor 3, TR3


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Tested Applications

Positive WB detected inHL-60 cells, PC-3 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25851-1-AP targets NR4A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR4A1 fusion protein Ag23088 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 4, group A, member 1
Calculated molecular weight 598 aa, 64 kDa
Observed molecular weight 64-68 kDa
GenBank accession numberBC016147
Gene symbol NR4A1
Gene ID (NCBI) 3164
RRIDAB_2880269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NR4A1 antibody 25851-1-APDownload protocol
IHC protocol for NR4A1 antibody 25851-1-APDownload protocol
IP protocol for NR4A1 antibody 25851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cancer Lett

NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway

Authors - Jinghan Zhu
mouseWB

FASEB J

The role of nuclear receptor 4A1 (NR4A1) in drug-induced gingival overgrowth.

Authors - Saki Hatano
mouseWB,IHC

J Cell Mol Med

NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression.

Authors - Dan-Dan Qin
  • KO Validated
ratIF

Phytomedicine

Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis.

Authors - Md Nazmul Haque
humanWB

Front Mol Biosci

Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.

Authors - Wenzhou Liu
  • KD Validated
mouseWB

Am J Physiol Endocrinol Metab

Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring.

Authors - Xiaoyan Wan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Angela (Verified Customer) (06-23-2020)

35ug total protein/lane1- Untransfected HEK2932- Transfection with human NR4A1 expression vector3- Transfection with rat NR4A1 expression vector

  • Applications: Western Blot
  • Primary Antibody Dilution: 500x
  • Cell Tissue Type: HEK293
NR4A1 Antibody Western Blot validation (500x dilution) in HEK293 (Cat no:25851-1-AP)