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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using NR4A3 Polyclonal antibody (55405-1-AP)

    WB analysis of mouse spleen using 55405-1-AP (same clone as 55405-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NR4A3 Polyclonal antibody (55405-1-AP)

    WB analysis using 55405-1-AP (same clone as 55405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using NR4A3 Polyclonal antibody (55405-1-AP)

    WB analysis of mouse thymus using 55405-1-AP (same clone as 55405-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ROS1728 cells using NR4A3 Polyclonal antibody (55405-1-AP)

    IF Staining of ROS1728 using 55405-1-AP (same clone as 55405-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed ROS1728 cells using NR4A3 antibody (55405-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

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NR4A3 Polyclonal antibody, PBS Only

NR4A3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 55405-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TEC, NOR1, MINOR, CSMF, CHN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55405-1-PBS targets NR4A3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 4, group A, member 3
Calculated molecular weight 70 kDa
Observed molecular weight 68 kDa
GenBank accession numberNM_006981
Gene Symbol NR4A3
Gene ID (NCBI) 8013
RRIDAB_2881322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92570
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear receptor 4A3 (NR4A3, also known as NOR-1), NR4A1 and NR4A2 are TFs belonging to NR4A orphan nuclear receptor subfamily. The role of all three NR4A members in regulating gene expression involved in cell proliferation, differentiation, apoptosis, inflammation and metabolism in cardiovascular diseases has been widely studied. In osteoblasts, NR4A receptor has been proved to regulate the expression and differentiation of osteoblast genes. It is reported that NR4A receptor is highly expressed in human coronary atherosclerotic lesions, and NR4A3 deficiency can reduce the proliferation and migration of VSMCs and the formation of neointima after vascular injury. Therefore, NR4A3 may play an important role in mediating phenotypic transformation of VSMC (PMID: 38629274). It is expected to be located in nucleus. The protein has three isforms, isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta. The calculated molecular weight of NR4A3 is 68 kDa.