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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRAP Polyclonal antibody (25977-1-AP)

    WB analysis using 25977-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NRAP Polyclonal antibody (25977-1-AP)

    WB analysis using 25977-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NRAP Polyclonal antibody (25977-1-AP)

    IHC staining of mouse skeletal muscle using 25977-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NRAP Polyclonal antibody (25977-1-AP)

    IHC staining of mouse skeletal muscle using 25977-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NRAP Polyclonal antibody

NRAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25977-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

N RAP, NRAP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25977-1-AP targets NRAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRAP fusion protein Ag23254 相同性解析による交差性が予測される生物種
Full Name nebulin-related anchoring protein
Observed molecular weight190-200 kDa
GenBank accession numberBC126407
Gene Symbol NRAP
Gene ID (NCBI) 4892
RRIDAB_2918098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRAP (Nebulin-related-anchoring protein) is a striated muscle-specific protein that is concentrated at intercalated disks in cardiac muscle and at myotendinous junctions in skeletal muscle. NRAP is a 197 kDa multidomain scaffolding protein from nebulin family with many interation partners such as α-actinin, actin, vinculin and filamin (PMID:21276443). During cardiomyocyte development in the foetal heart, NRAP is involved in myofibrillar assembly and links terminal actin filaments to membrane complexes in the adult heart. Several studies observed that NRAP mutation would contributed to the dilated cardiomyopathy which is a highly heterogeneous disease with almost 100 candidates genes to date (PMID:28611399).

Protocols

Product Specific Protocols
WB protocol for NRAP antibody 25977-1-APDownload protocol
IHC protocol for NRAP antibody 25977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols