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  • Featured Product
  • KD/KO Validated

NRAS Polyclonal antibody

NRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10724-1-AP

Synonyms

HRAS1, Transforming protein N Ras, NRAS1, N ras, GTPase NRas

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NRAS Polyclonal antibody (10724-1-AP)

    WB analysis using 10724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NRAS Polyclonal antibody (10724-1-AP)

    WB analysis of Jurkat using 10724-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using NRAS Polyclonal antibody (10724-1-AP)

    WB analysis of C6 using 10724-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using NRAS Polyclonal antibody (10724-1-AP)

    WB analysis of NIH/3T3 using 10724-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NRAS Polyclonal antibody (10724-1-AP)

    IHC staining of human pancreas using 10724-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NRAS Polyclonal antibody (10724-1-AP)

    IHC staining of human pancreas using 10724-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NRAS Polyclonal antibody (10724-1-AP)

    IHC staining of human pancreas using 10724-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NRAS Polyclonal antibody (10724-1-AP)

    IHC staining of human pancreas using 10724-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NRAS Polyclonal antibody (10724-1-AP)

    IHC staining of human testis using 10724-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NRAS Polyclonal antibody (10724-1-AP)

    IHC staining of human testis using 10724-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NRAS Polyclonal antibody (10724-1-AP)

    IF Staining of HeLa using 10724-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NRAS antibody (10724-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using NRAS Polyclonal antibody (10724-1-AP)

    FC experiment of HepG2 using 10724-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NRAS (10724-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, C6 cells, NIH/3T3 cells, Jurkat cells, A549 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman pancreas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10724-1-AP targets NRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRAS fusion protein Ag1081 相同性解析による交差性が予測される生物種
Full Name neuroblastoma RAS viral (v-ras) oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC005219
Gene symbol NRAS
Gene ID (NCBI) 4893
RRIDAB_2154209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML).

Protocols

Product Specific Protocols
WB protocol for NRAS antibody 10724-1-APDownload protocol
IHC protocol for NRAS antibody 10724-1-APDownload protocol
IF protocol for NRAS antibody 10724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels.

Authors - Xue Hao
View Article
humanWB

Gut

RNA helicase DDX5 enables STAT1 mRNA translation and interferon signalling in hepatitis B virus replicating hepatocytes.

Authors - Jiazeng Sun
View Article
humanWB

Nat Commun

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

Authors - Perla Pucci
View Article
humanIF

Nat Commun

KRAS regulation by small non-coding RNAs and SNARE proteins.

Authors - Yonglu Che
View Article
human，mouseWB

Biomaterials

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Authors - Yuanyuan Liu
View Article
humanWB

Clin Cancer Res

Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.

Authors - Chen Liu
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Brandon (Verified Customer) (11-27-2018)

Worked excellent on mouse tissue from inducible tumor model!

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse Tumor Tissue