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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NRCAM Recombinant antibody (84690-1-RR)

    WB analysis of SH-SY5Y using 84690-1-RR (same clone as 84690-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NRCAM Recombinant antibody (84690-1-RR)

    WB analysis using 84690-1-RR (same clone as 84690-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84690-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84690-1-RR

    Affinity and Kinetic Characterization of 84690-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84690-1-RR against Human NRCAM were performed. The affinity constant is below 1 pM.

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NRCAM Recombinant antibody, PBS Only

NRCAM Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84690-1-PBS
Clone No.241862G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Ng CAM related, Neuronal surface protein Bravo, neuronal cell adhesion molecule, KIAA0343, hBravo

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84690-1-PBS targets NRCAM in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRCAM fusion protein Ag16237 相同性解析による交差性が予測される生物種
Full Name neuronal cell adhesion molecule
Calculated molecular weight 1304 aa, 144 kDa
Observed molecular weight144 kDa
GenBank accession numberBC098401
Gene Symbol NRCAM
Gene ID (NCBI) 4897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92823
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).