Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15630-1-AP targets NRD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NRD1 fusion protein Ag7923 相同性解析による交差性が予測される生物種 |
| Full Name | nardilysin (N-arginine dibasic convertase) |
| Calculated molecular weight | 132 kDa |
| Observed molecular weight | 135-140 kDa |
| GenBank accession number | BC008775 |
| Gene Symbol | NRD1 |
| Gene ID (NCBI) | 4898 |
| RRID | AB_2154525 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43847 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NRD1(Nardilysin) is also named as NRD-C(N-arginine dibasic convertase). It is a zinc metalloendopeptidase of the M16 pitrilysin family that cleaves peptides at dibasic residues. The enzyme has also been found in neural tissues of mouse embryos indicating a role in neural development. Although nardilysin is primarily found in the cytosol, it has been reported that nardilysin is located on the cell surface acting as a receptor for heparinbinding EGF-like growth factor(PMID:15809022). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NRD1 antibody 15630-1-AP | Download protocol |
| IHC protocol for NRD1 antibody 15630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Diabetes Nardilysin-dependent proteolysis of cell-associated VTCN1 (B7-H4) marks type 1 diabetes development. | ||
J Immunol Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes. | ||
J Clin Med Serum Nardilysin as a Prognostic Biomarker in Pancreatic Ductal Adenocarcinoma. |