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NRF1/nuclear respiratory factor 1 Polyclonal antibody

NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12482-1-AP

Synonyms

NRF1, nuclear respiratory factor 1, NRF-1, NRF 1, Alpha-pal


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Tested Applications

Positive WB detected inA549 cells, human lung tissue, HEK-293T cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish, goat, ducks
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag3172 相同性解析による交差性が予測される生物種
Full Name nuclear respiratory factor 1
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 67 kDa, 45 kDa
GenBank accession numberBC016925
Gene symbol NRF1
Gene ID (NCBI) 4899
RRIDAB_2282876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Protocols

Product Specific Protocols
WB protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-APDownload protocol
IHC protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-APDownload protocol
IP protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
chickenWB,IHC

J Hazard Mater

Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers

Authors - Gaolong Zhong
WB

J Autoimmun

Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis

Authors - Jean-Baptiste Vulsteke
humanWB

Redox Biol

Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.

Authors - Hao Wu
chickenWB

Cell Commun Signal

Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation

Authors - Bowen Hu
humanWB

Mater Today Bio

Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

Authors - Zhanqiu Dai