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  • KD/KO Validated

GABPA Polyclonal antibody

GABPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21542-1-AP

Synonyms

NFT2, GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A


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Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, mouse liver tissue, MCF-7 cells, A549 cells, rat brain tissue, K-562 cells, NIH/3T3 cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21542-1-AP targets GABPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABPA fusion protein Ag16191 相同性解析による交差性が予測される生物種
Full Name GA binding protein transcription factor, alpha subunit 60kDa
Calculated molecular weight 454 aa, 51 kDa
Observed molecular weight 56-60 kDa
GenBank accession numberBC035031
Gene symbol GABPA
Gene ID (NCBI) 2551
RRIDAB_10858481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Protocols

Product Specific Protocols
WB protocol for GABPA antibody 21542-1-APDownload protocol
IHC protocol for GABPA antibody 21542-1-APDownload protocol
IF protocol for GABPA antibody 21542-1-APDownload protocol
IP protocol for GABPA antibody 21542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Hepatology

High mobility group protein B1 controls liver cancer initiation through yes-associated protein -dependent aerobic glycolysis.

Authors - Ruochan Chen
human, mouseWB,IHC,IF

Cell Rep

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Authors - Wu Hongtan H
  • KD Validated
mouseWB

Cardiovasc Res

Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oestrogen receptor beta.

Authors - Elke Dworatzek
mouseWB

Stem Cells

MZF1 and GABP Cooperate with Sox2 in Regulating the Expression of YAP1 in Cancer Cells.

Authors - Narendra Kumar Verma
  • KD Validated
humanWB

Biology (Basel)

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.

Authors - Yanbing Wang
humanWB,IHC

BMC Cancer

GABPA predicts prognosis and inhibits metastasis of hepatocellular carcinoma.

Authors - Sheng Zhang
  • KD Validated