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GABPA Polyclonal antibody

Name: GABPA Polyclonal antibody
Brand: Proteintech
SKU: 21542-1-AP

GABPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21542-1-AP

Synonyms

NFT2, GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 21542-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 21542-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 21542-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21542-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21542-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 21542-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21542-1-AP
IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.
IP experiment of HeLa using 21542-1-AP
IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human breast cancer using 21542-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 21542-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 21542-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 21542-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 21542-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GABPA antibody (21542-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 21542-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, mouse liver tissue, MCF-7 cells, A549 cells, rat brain tissue, K-562 cells, NIH/3T3 cells, mouse brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human cervical cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

21542-1-AP targets GABPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GABPA fusion protein Ag16191 相同性解析による交差性が予測される生物種
Full Name	GA binding protein transcription factor, alpha subunit 60kDa
Calculated molecular weight	454 aa, 51 kDa
Observed molecular weight	56-60 kDa
GenBank accession number	BC035031
Gene symbol	GABPA
Gene ID (NCBI)	2551
RRID	AB_10858481
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Protocols

Product Specific Protocols
WB protocol for GABPA antibody 21542-1-AP	Download protocol
IHC protocol for GABPA antibody 21542-1-AP	Download protocol
IF protocol for GABPA antibody 21542-1-AP	Download protocol
IP protocol for GABPA antibody 21542-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Hepatology High mobility group protein B1 controls liver cancer initiation through yes-associated protein -dependent aerobic glycolysis. Authors - Ruochan Chen View Article
human, mouse	WB,IHC,IF	Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Authors - Wu Hongtan H KD Validated View Article
mouse	WB	Cardiovasc Res Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oestrogen receptor beta. Authors - Elke Dworatzek View Article
mouse	WB	Stem Cells MZF1 and GABP Cooperate with Sox2 in Regulating the Expression of YAP1 in Cancer Cells. Authors - Narendra Kumar Verma KD Validated View Article
human	WB	Biology (Basel) TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis. Authors - Yanbing Wang View Article
human	WB,IHC	BMC Cancer GABPA predicts prognosis and inhibits metastasis of hepatocellular carcinoma. Authors - Sheng Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABPA Polyclonal antibody

GABPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 21542-1-AP

WB analysis of mouse liver using 21542-1-AP

WB analysis of rat brain using 21542-1-AP

WB analysis of MCF-7 using 21542-1-AP

WB analysis of A431 using 21542-1-AP

WB analysis of A549 using 21542-1-AP

IP experiment of HeLa using 21542-1-AP

IP experiment of HeLa using 21542-1-AP

IHC staining of human breast cancer using 21542-1-AP

IHC staining of human breast cancer using 21542-1-AP

IHC staining of human cervical cancer using 21542-1-AP

IHC staining of human cervical cancer using 21542-1-AP

IF Staining of HEK-293 using 21542-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

High mobility group protein B1 controls liver cancer initiation through yes-associated protein -dependent aerobic glycolysis.

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oestrogen receptor beta.

MZF1 and GABP Cooperate with Sox2 in Regulating the Expression of YAP1 in Cancer Cells.

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.

GABPA predicts prognosis and inhibits metastasis of hepatocellular carcinoma.