NRG1, isoform SMDF Polyclonal antibody

Name: NRG1, isoform SMDF Polyclonal antibody
Brand: Proteintech
SKU: 10527-1-AP

NRG1, isoform SMDF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10527-1-AP

Synonyms

NRG1, NRG1A, HGL, Heregulin, Glial growth factor

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 10527-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10527-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse stomach using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 1x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10527-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10527-1-AP

Size/Concentration

ご購入について

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected in	mouse stomach tissue, human stomach tissue, mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IHC	See 4 publications below
IF	See 5 publications below

Product Information

10527-1-AP targets NRG1, isoform SMDF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NRG1, isoform SMDF fusion protein Ag0803 相同性解析による交差性が予測される生物種
Full Name	neuregulin 1
Calculated molecular weight	70 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC007675
Gene symbol	NRG1
Gene ID (NCBI)	3084
RRID	AB_2154659
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. Studies has been reportrd, the full-length type III NRG1 (NRG1 isoform SMDF) is more than 110 kDa, the 70-75 kDa band is a membrane bound form and it can be cleaved by an unknown enzyme releases the ∼45 kDa secreted form of NRG1(PMID: 21047912, PMID: 11425901). The immunogen of this antibody is against NRG1 isoform SMDF.

Protocols

Product Specific Protocols
WB protocol for NRG1, isoform SMDF antibody 10527-1-AP	Download protocol
IHC protocol for NRG1, isoform SMDF antibody 10527-1-AP	Download protocol
IF protocol for NRG1, isoform SMDF antibody 10527-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	ACS Biomater Sci Eng Sericin Nerve Guidance Conduit Delivering Therapeutically Repurposed Clobetasol for Functional and Structural Regeneration of Transected Peripheral Nerves Authors - Lei Zhang View Article
mouse	WB,IHC,IF	Ecotoxicol Environ Saf Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice. Authors - Dongmin Yu View Article
human	IHC	J Exp Clin Cancer Res miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling. Authors - Dong-Min Shi View Article
mouse	WB	Am J Physiol Renal Physiol ErbB4 deletion accelerates renal fibrosis following renal injury. Authors - Fenghua Zeng View Article
human	IF	Folia Neuropathol miR-143-3p inhibition promotes neuronal survival in an Alzheimer's disease cell model by targeting neuregulin-1. Authors - Chengyan Sun View Article
mouse	WB	Evid Based Complement Alternat Med Extracellular Vesicle-Encapsulated miR-183-5p from Rhynchophylline-Treated H9c2 Cells Protect against Methamphetamine-Induced Dependence in Mouse Brain by Targeting NRG1. Authors - Yuting Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRG1, isoform SMDF Polyclonal antibody

NRG1, isoform SMDF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 10527-1-AP

WB analysis of SH-SY5Y using 10527-1-AP

IHC staining of mouse stomach using 10527-1-AP

IHC staining of mouse stomach using 10527-1-AP

IHC staining of human stomach using 10527-1-AP

IHC staining of human stomach using 10527-1-AP

IHC staining of mouse cerebellum using 10527-1-AP

IHC staining of mouse cerebellum using 10527-1-AP

IHC staining of mouse cerebellum using 10527-1-AP

IHC staining of mouse cerebellum using 10527-1-AP

IF Staining of MCF-7 using 10527-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Sericin Nerve Guidance Conduit Delivering Therapeutically Repurposed Clobetasol for Functional and Structural Regeneration of Transected Peripheral Nerves

Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice.

miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling.

ErbB4 deletion accelerates renal fibrosis following renal injury.

miR-143-3p inhibition promotes neuronal survival in an Alzheimer's disease cell model by targeting neuregulin-1.

Extracellular Vesicle-Encapsulated miR-183-5p from Rhynchophylline-Treated H9c2 Cells Protect against Methamphetamine-Induced Dependence in Mouse Brain by Targeting NRG1.