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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRG1, isoform Alpha Polyclonal antibody

NRG1, isoform Alpha Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31043-1-AP

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform Alpha, NRG1A, Pro NRG1, SMDF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRG1, isoform Alpha Polyclonal antibody (31043-1-AP)

    WB analysis using 31043-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31043-1-AP (NRG1, isoform Alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NRG1, isoform Alpha Polyclonal antibody (31043-1-AP)

    IF Staining of MCF-7 using 31043-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Alpha antibody (31043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, A549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31043-1-AP targets NRG1, isoform Alpha in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRG1, isoform Alpha fusion protein Ag35114 相同性解析による交差性が予測される生物種
Full Name neuregulin 1
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007675
Gene symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.

Protocols

Product Specific Protocols
WB protocol for NRG1, isoform Alpha antibody 31043-1-APDownload protocol
IF protocol for NRG1, isoform Alpha antibody 31043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols