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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRG1, isoform Alpha Recombinant antibody

NRG1, isoform Alpha Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240067A6

Cat no : 83251-2-RR

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, Pro NRG1, SMDF

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDA-MB-231 cells using NRG1, isoform Alpha Recombinant antibody (83251-2-RR)

    IF Staining of MDA-MB-231 using 83251-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MDA-MB-231 cells using NRG1, isoform Alpha Recombinant antibody (83251-2-RR)

    IF Staining of MDA-MB-231 using 83251-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity experiments of 83251-2-RR

    Affinity experiments of 83251-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83251-2-RR against Human NRG1, isoform Alpha were performed. The affinity constant is 10.7 nM.

Tested Applications

Positive IF detected inMDA-MB-231 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83251-2-RR targets NRG1, isoform Alpha in IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRG1, isoform Alpha fusion protein Ag35114 相同性解析による交差性が予測される生物種
Full Name neuregulin 1
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007675
Gene symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.

Protocols

Product Specific Protocols
IF protocol for NRG1, isoform Alpha antibody 83251-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols