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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRG1, isoform SMDF Recombinant antibody

NRG1, isoform SMDF Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240184G2

Cat no : 83323-6-RR

Synonyms

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NRG1, isoform SMDF Recombinant antibody (83323-6-RR)

    IHC staining of mouse cerebellum using 83323-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NRG1, isoform SMDF Recombinant antibody (83323-6-RR)

    IHC staining of mouse cerebellum using 83323-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NRG1, isoform SMDF Recombinant antibody (83323-6-RR)

    IF Staining of rat brain using 83323-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NRG1, isoform SMDF Recombinant antibody (83323-6-RR)

    IF Staining of rat brain using 83323-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NRG1, isoform SMDF Recombinant antibody (83323-6-RR)

    IF Staining of MCF-7 using 83323-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83323-6-RR

    Affinity and Kinetic Characterization of 83323-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83323-6-RR against Human NRG1, isoform SMDF were performed. The affinity constant is 1.64 nM.

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Tested Applications

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83323-6-RR is NRG1, isoform SMDF Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRG1, isoform SMDF fusion protein Ag0803 相同性解析による交差性が予測される生物種
Full Name neuregulin 1
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007675
Gene symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.

Protocols

Product Specific Protocols
IHC protocol for NRG1, isoform SMDF antibody 83323-6-RRDownload protocol
IF protocol for NRG1, isoform SMDF antibody 83323-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols