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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRG2 Polyclonal antibody (20690-1-AP)

    WB analysis using 20690-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20690-1-AP (NRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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NRG2 Polyclonal antibody

NRG2 Polyclonal Antibody for WB, ELISA
Cat No. 20690-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Divergent of neuregulin 1, Don 1, HRG2, neuregulin 2, NRG 2, NRG2, NTAK, Pro NRG2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20690-1-AP targets NRG2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name neuregulin 2
Calculated molecular weight 92 kDa
Observed molecular weight46 kDa
GenBank accession numberNM_004883
Gene Symbol NRG2
Gene ID (NCBI) 9542
RRIDAB_3085611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRG2 is a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors, this protein induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1, another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease.

Protocols

Product Specific Protocols
WB protocol for NRG2 antibody 20690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols