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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRG4 Polyclonal antibody

NRG4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 27292-1-AP

Synonyms

HRG4, neuregulin 4, NRG 4, NRG4, Pro NRG4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRG4 Polyclonal antibody (27292-1-AP)

    WB analysis using 27292-1-AP

    Recombinant human NRG4 protein Eg0997 was subjected to SDS PAGE followed by western blot with 27292-1-AP (NRG4 antibody) at dilution of 1:3000 and incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NRG4 Polyclonal antibody (27292-1-AP)

    IHC staining of human prostate cancer using 27292-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27292-1-AP (NRG4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NRG4 Polyclonal antibody (27292-1-AP)

    IHC staining of human prostate cancer using 27292-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27292-1-AP (NRG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NRG4 Polyclonal antibody (27292-1-AP)

    IHC staining of human breast cancer using 27292-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27292-1-AP (NRG4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NRG4 Polyclonal antibody (27292-1-AP)

    IHC staining of human breast cancer using 27292-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27292-1-AP (NRG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27292-1-AP targets NRG4 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRG4 fusion protein Ag26044 相同性解析による交差性が予測される生物種
Full Name neuregulin 4
Calculated molecular weight 13 kDa
GenBank accession numberBC017568
Gene symbol NRG4
Gene ID (NCBI) 145957
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuregulin 4 (Nrg4), a member of the epidermal growth factor (EGF) family of extracellular ligands, is expressed in multiple organs with the highest expression levels in brown adipose tissue. Neuregulin 4 is a secreted adipokine recently identified as playing an important role in modulating systemic energy metabolism and in the development of metabolic disorders in rodent and human obesity, including type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). Nrg4 attenuates hepatic lipogenic signaling and preserves glucose and lipid homeostasis in obesity.

Protocols

Product Specific Protocols
WB protocol for NRG4 antibody 27292-1-APDownload protocol
IHC protocol for NRG4 antibody 27292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols