Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
10440-1-AP targets Neurogranin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Neurogranin fusion protein Ag0676 相同性解析による交差性が予測される生物種 |
| Full Name | neurogranin (protein kinase C substrate, RC3) |
| Calculated molecular weight | 7.6 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC002835 |
| Gene Symbol | Neurogranin |
| Gene ID (NCBI) | 4900 |
| RRID | AB_2251586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92686 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.The observed molecular weight of human NRGN by Western blotting is about 15-17 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Neurogranin antibody 10440-1-AP | Download protocol |
| IHC protocol for Neurogranin antibody 10440-1-AP | Download protocol |
| IP protocol for Neurogranin antibody 10440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cells Specificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure. | ||
Elife An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning. |