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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neurogranin Recombinant antibody (83593-5-RR)

    WB analysis using 83593-5-RR (same clone as 83593-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83593-5-RR (Neurogranin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurogranin Recombinant antibody (83593-5-RR)

    IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83593-5-RR (Neurogranin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurogranin Recombinant antibody (83593-5-RR)

    IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83593-5-RR (Neurogranin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using Neurogranin Recombinant antibody (83593-5-RR)

    FC experiment of A549 using 83593-5-RR (same clone as 83593-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neurogranin Recombinant antibody (83593-5-RR, Clone:240526B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83593-5-RR

    Affinity and Kinetic Characterization of 83593-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83593-5-RR against Human NRGN were performed. The affinity constant is 0.304 nM.

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Neurogranin Recombinant antibody, PBS Only

Neurogranin Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83593-5-PBS
Clone No.240526B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

NRGN, P17, Ng, NEUG(55-78), hng

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83593-5-PBS targets Neurogranin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Neurogranin fusion protein Ag0676 相同性解析による交差性が予測される生物種
Full Name neurogranin (protein kinase C substrate, RC3)
Calculated molecular weight 7.6 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC002835
Gene Symbol Neurogranin
Gene ID (NCBI) 4900
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92686
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.