Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
17574-1-AP targets NRSN2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NRSN2 fusion protein Ag10387 相同性解析による交差性が予測される生物種 |
| Full Name | neurensin 2 |
| Calculated molecular weight | 204 aa, 22 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC001963 |
| Gene Symbol | NRSN2 |
| Gene ID (NCBI) | 80023 |
| RRID | AB_2155379 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NRSN2 antibody 17574-1-AP | Download protocol |
| IHC protocol for NRSN2 antibody 17574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Cancer Res NRSN2 promotes osteosarcoma cell proliferation and growth through PI3K/Akt/MTOR and Wnt/β-catenin signaling.
| ||
Biomed Pharmacother Highly expressed NRSN2 is related to malignant phenotype in ovarian cancer.
| ||
Dig Dis Sci Down-Regulated NRSN2 Promotes Cell Proliferation and Survival Through PI3K/Akt/mTOR Pathway in Hepatocellular Carcinoma. | ||
Int J Clin Exp Pathol NRSN2 promotes non-small cell lung cancer cell growth through PI3K/Akt/mTOR pathway. |