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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using NSBP1 Polyclonal antibody (23955-1-AP)

    WB analysis of PC-3 using 23955-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NSBP1 Polyclonal antibody (23955-1-AP)

    WB analysis of HeLa using 23955-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NSBP1 Polyclonal antibody (23955-1-AP)

    WB analysis of HepG2 using 23955-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSBP1 Polyclonal antibody (23955-1-AP)

    IF Staining of HepG2 using 23955-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSBP1 antibody (23955-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NSBP1 Polyclonal antibody

NSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23955-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nucleosome-binding protein 1, NSBP 1, HMGN5, High mobility group nucleosome-binding domain-containing protein 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23955-1-AP targets NSBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSBP1 fusion protein Ag20884 相同性解析による交差性が予測される生物種
Full Name nucleosomal binding protein 1
Calculated molecular weight 282 aa, 32 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005342
Gene Symbol NSBP1
Gene ID (NCBI) 79366
RRIDAB_2879374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP82970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSBP1, also named Nucleosome-binding protein 1 or HMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596).

Protocols

Product Specific Protocols
WB protocol for NSBP1 antibody 23955-1-APDownload protocol
IHC protocol for NSBP1 antibody 23955-1-APDownload protocol
IF protocol for NSBP1 antibody 23955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur Rev Med Pharmacol Sci

HMGN5 promotes invasion and migration of colorectal cancer through activating FGF/FGFR pathway.

Authors - G-J Zhu
View Article