• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSD1 Polyclonal antibody

NSD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27346-1-AP

Synonyms

ARA267, H3 K36 HMTase, H4 K20 HMTase, KMT3B, Lysine N methyltransferase 3B, NSD1, SOTOS, STO

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NSD1 Polyclonal antibody (27346-1-AP)

    WB analysis using 27346-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27346-1-AP (NSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27346-1-AP targets NSD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSD1 fusion protein Ag26425 相同性解析による交差性が予測される生物種
Full Name nuclear receptor binding SET domain protein 1
Observed molecular weight267-310 kDa
GenBank accession numberBC150628
Gene symbol NSD1
Gene ID (NCBI) 64324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSD1 (nuclear receptor SET (su(var)3-9, enhancer-of-zeste,trithorax) domain containing protein-1), which is isolated and characterized in 2001, belongs to the NSD protein lysine methyltransferase (KMT) family. This family has three members, NSD1 (KMT3B), NSD2 (WHSC1/MMSET) and NSD3 (WHSC1L1), which could regulate the expression of target genes through methylation of lysine 36 on histone H3(H3K36). NSD1 could bind various promoter elements to regulate transcription via interactions with H3K36 methylation and RNA polymerase II.

Protocols

Product Specific Protocols
WB protocol for NSD1 antibody 27346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols