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Tested Applications
| Positive WB detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27346-1-AP targets NSD1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NSD1 fusion protein Ag26425 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear receptor binding SET domain protein 1 |
| Observed molecular weight | 267-310 kDa |
| GenBank accession number | BC150628 |
| Gene Symbol | NSD1 |
| Gene ID (NCBI) | 64324 |
| RRID | AB_3085948 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96L73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSD1 (nuclear receptor SET (su(var)3-9, enhancer-of-zeste,trithorax) domain containing protein-1), which is isolated and characterized in 2001, belongs to the NSD protein lysine methyltransferase (KMT) family. This family has three members, NSD1 (KMT3B), NSD2 (WHSC1/MMSET) and NSD3 (WHSC1L1), which could regulate the expression of target genes through methylation of lysine 36 on histone H3(H3K36). NSD1 could bind various promoter elements to regulate transcription via interactions with H3K36 methylation and RNA polymerase II.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSD1 antibody 27346-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |