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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSD3 Recombinant antibody

NSD3 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230499C5

Cat no : 83116-1-RR

Synonyms

WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using NSD3 Recombinant antibody (83116-1-RR)

    IHC staining of human lung squamous cell carcinoma using 83116-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-1-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NSD3 Recombinant antibody (83116-1-RR)

    IF Staining of MCF-7 using 83116-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NSD3 antibody (83116-1-RR, Clone: 230499C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using NSD3 Recombinant antibody (83116-1-RR)

    FC experiment of HeLa using 83116-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human NSD3 (83116-1-RR, Clone:230499C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83116-1-RR

    Affinity and Kinetic Characterization of 83116-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83116-1-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IHC detected inhuman lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83116-1-RR targets NSD3 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSD3 fusion protein Ag1894 相同性解析による交差性が予測される生物種
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Calculated molecular weight 1437 aa, 162 kDa
GenBank accession numberBC012059
Gene symbol WHSC1L1
Gene ID (NCBI) 54904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for NSD3 antibody 83116-1-RRDownload protocol
IF protocol for NSD3 antibody 83116-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols