NSE Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

WB result of NSE antibody (66150-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

rat brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

C6 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

pig brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NSE (66150-1-Ig, Clone:6F8G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.