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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSF Polyclonal antibody

NSF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21172-1-AP

Synonyms

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSF Polyclonal antibody (21172-1-AP)

    WB analysis using 21172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21172-1-AP (NSF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NSF Polyclonal antibody (21172-1-AP)

    IP experiment of mouse brain using 21172-1-AP

    IP result of anti-NSF (IP:21172-1-AP, 4ug; Detection:21172-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NSF Polyclonal antibody (21172-1-AP)

    IHC staining of mouse brain using 21172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21172-1-AP (NSF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NSF Polyclonal antibody (21172-1-AP)

    IHC staining of mouse brain using 21172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21172-1-AP (NSF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

21172-1-AP targets NSF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSF fusion protein Ag15422 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor
Calculated molecular weight 744 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC030613
Gene symbol NSF
Gene ID (NCBI) 4905
RRIDAB_2878823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The N-ethylmaleimide-sensitive fusion protein (NSF) is a 70-83 kDa cytosol protein that is expressed in all cell types. NSF functions as an ATPase and it is a part of the 20S membrane fusion apparatus with the vesicular SNAP receptor (v-SNARE) synaptobrevin, and the target SNAREs (t-SNARE) syntaxin and SNAP-25. With the hydrolysis of ATP by NSF, the released energy may set-up or drive membrane fusion processes. NSF is required for general membrane transport and trafficking.

Protocols

Product Specific Protocols
WB protocol for NSF antibody 21172-1-APDownload protocol
IHC protocol for NSF antibody 21172-1-APDownload protocol
IP protocol for NSF antibody 21172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Death Differ

Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.

Authors - Zhaokang Cui
View Article