NSF Polyclonal antibody
NSF Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21172-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
21172-1-AP targets NSF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NSF fusion protein Ag15422 相同性解析による交差性が予測される生物種 |
Full Name | N-ethylmaleimide-sensitive factor |
Calculated molecular weight | 744 aa, 83 kDa |
Observed molecular weight | 83 kDa |
GenBank accession number | BC030613 |
Gene symbol | NSF |
Gene ID (NCBI) | 4905 |
RRID | AB_2878823 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The N-ethylmaleimide-sensitive fusion protein (NSF) is a 70-83 kDa cytosol protein that is expressed in all cell types. NSF functions as an ATPase and it is a part of the 20S membrane fusion apparatus with the vesicular SNAP receptor (v-SNARE) synaptobrevin, and the target SNAREs (t-SNARE) syntaxin and SNAP-25. With the hydrolysis of ATP by NSF, the released energy may set-up or drive membrane fusion processes. NSF is required for general membrane transport and trafficking.
Protocols
Product Specific Protocols | |
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WB protocol for NSF antibody 21172-1-AP | Download protocol |
IHC protocol for NSF antibody 21172-1-AP | Download protocol |
IP protocol for NSF antibody 21172-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Differ Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy. |