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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

NSF Monoclonal antibody, PBS Only

NSF Monoclonal Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rabbit, Rat, Mouse, Chicken

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B6H6

Cat no : 68061-1-PBS

Synonyms

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis using 68061-1-Ig (same clone as 68061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis of PC-12 using 68061-1-Ig (same clone as 68061-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis of SH-SY5Y using 68061-1-Ig (same clone as 68061-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis of HEK-293 using 68061-1-Ig (same clone as 68061-1-PBS)

    WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NSF Monoclonal antibody (68061-1-Ig)

    IF Staining of mouse brain using 68061-1-Ig (same clone as 68061-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68061-1-PBS targets NSF in WB, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse, Chicken samples.

Tested Reactivity Human, Pig, Rabbit, Rat, Mouse, Chicken
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NSF fusion protein Ag15422 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor
Calculated molecular weight 744 aa, 83 kDa
Observed molecular weight70-83 kDa
GenBank accession numberBC030613
Gene symbol NSF
Gene ID (NCBI) 4905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.