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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSMAF Polyclonal antibody

NSMAF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19407-1-AP

Synonyms

Protein FAN, FAN, Factor associated with N-SMase activation

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSMAF Polyclonal antibody (19407-1-AP)

    WB analysis using 19407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19407-1-AP (NSMAF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NSMAF Polyclonal antibody (19407-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 19407-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19407-1-AP (NSMAF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NSMAF Polyclonal antibody (19407-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 19407-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19407-1-AP (NSMAF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, U2OS cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19407-1-AP targets NSMAF in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSMAF fusion protein Ag5730 相同性解析による交差性が予測される生物種
Full Name neutral sphingomyelinase (N-SMase) activation associated factor
Calculated molecular weight 104 kDa
Observed molecular weight100-104 kDa
GenBank accession numberBC041124
Gene symbol NSMAF
Gene ID (NCBI) 8439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSMAF is a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation.

Protocols

Product Specific Protocols
WB protocol for NSMAF antibody 19407-1-APDownload protocol
IHC protocol for NSMAF antibody 19407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols