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  • Featured Product
  • KD/KO Validated

NSMCE2 Polyclonal antibody

NSMCE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13627-1-AP

Synonyms

C8orf36, E3 SUMO protein ligase NSE2, hMMS21, MMS21, MMS21 homolog, Non SMC element 2 homolog, NSE2, NSMCE2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSMCE2 Polyclonal antibody (13627-1-AP)

    WB analysis using 13627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13627-1-AP (NSMCE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NSMCE2 Polyclonal antibody (13627-1-AP)

    WB analysis of HeLa using 13627-1-AP

    WB result of NSMCE2 antibody (13627-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSMCE2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NSMCE2 Polyclonal antibody (13627-1-AP)

    IHC staining of mouse testis using 13627-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13627-1-AP (NSMCE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using NSMCE2 Polyclonal antibody (13627-1-AP)

    IHC staining of mouse ovary using 13627-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13627-1-AP (NSMCE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, U2OS cells
Positive IHC detected inmouse testis tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13627-1-AP targets NSMCE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSMCE2 fusion protein Ag4545 相同性解析による交差性が予測される生物種
Full Name non-SMC element 2, MMS21 homolog (S. cerevisiae)
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC032797
Gene symbol NSMCE2
Gene ID (NCBI) 286053
RRIDAB_10637854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NSMCE2 antibody 13627-1-APDownload protocol
IHC protocol for NSMCE2 antibody 13627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu
View Article